极地研究

极地研究 ›› 2021, Vol. 33 ›› Issue (2): 241-249.DOI: 10. 13679/j.jdyj.20200045

• 研究论文 • 上一篇    下一篇

北极新奥尔松地区藻类可培养附生细菌多样性研究

孙晓梦1,2; 樊守金1   

  1. 1山东师范大学生命科学学院, 山东 济南 250014; 2山东大学微生物技术国家重点实验室, 山东 青岛 266237
  • 收稿日期:2020-05-18 修回日期:2020-07-24 出版日期:2021-06-30 发布日期:2021-05-31
  • 通讯作者: 樊守金
  • 基金资助:
    国家自然科学基金(31800185)

Diversity of cultivable epiphytic bacteria of  Polysiphonia algae from Ny-Ålesund, Svaldbard, Arctic

Sun Xiaomeng1,2, Fan Shoujin1   

  1. 1 College of Life Sciences, Shangdong Normal University, Jinan 250014, China;  2 State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China
  • Received:2020-05-18 Revised:2020-07-24 Online:2021-06-30 Published:2021-05-31
  • Contact: Fan Shoujin

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摘要： 为研究北极地区海洋藻类可培养附生细菌的多样性, 利用稀释平板法自2019年7月采集的北极新奥尔松地区的海洋红藻多管藻(Polysiphonia sp.)样品中分离纯化得到60株可培养的藻类附生细菌。16S rRNA基因序列测定分析表明所分离的藻类附生细菌主要隶属于γ-变形菌纲(39株, 65%), 其次隶属于放线菌纲(10株, 17%)和黄杆菌纲(8株, 13%), 仅有少量隶属于α-变形菌纲(2株, 3%)和芽孢杆菌纲(1株, 2%), 其中的优势细菌属为Psychrobacter属(22株, 37%)和Pseudoalteromonas属(12株, 20%)。此次获得了较多极地藻类多管藻的附生细菌菌株, 初步揭示了多管藻可培养附生细菌的多样性, 为多管藻自身以及其附生细菌生态功能及应用开发研究奠定了基础。

关键词: 北极藻类, 多管藻, 附生细菌, 16S rRNA 基因, 多样性

Abstract: To analyze the diversity of epiphytic bacteria in Arctic algae, a red algae sample (Polysiphonia sp.) was collected from Ny-Ålesund, Svaldbard, Arctic in July 2019. A total of 60 cultivable strains of epiphytic bacteria were isolated from the sample using the dilution plating method. Analysis of 16S rRNA gene sequences revealed that the isolated strains mainly belonged to the class γ-Proteobacteria (39 strains, 65% of the total strains isolated from the red algae samples), followed by the classes Actinobacteria (10 strains, 17%), Flavobacteriia (eight strains, 13%), α-Proteobacteria (two strains, 3%) and Bacilli (one strain, 2%). The strains were affiliated with 12 genera with the predominant genera being Psychrobacter (22 strains, 37%) and Pseudoalteromonas (12 strains, 20%). The results preliminarily illustrate the diversity of cultivable epiphytic bacteria in Arctic red algae (Polysiphonia sp.), laying a foundation for studies on the ecological functions and exploitation of these particular Arctic microbial resources.

Key words: Arctic algae, Polysiphonia sp., epiphytic bacteria, 16S rRNA gene, diversity